[Effect of hypothermia on activities of tubule motor protein and constructure protein after cerebral ischemia in gerbils].

ABSTRACT

AIM: To study the effect of hypothermia on kinesin and MAP2 activities after cerebral ischemia in gerbils, and investigate the relationship between changes of their activities and DND. METHODS: Immunoreactivity of kinesin and MAP2 were determined by immunohistochemical study in combination with computer image analysis system, and DND were assayed by histological examination. RESULTS: Hypothermia significantly reduced DND after cerebral ischemia. Immunoreactivities of MAP2 and kinesin progressively decreased during forebrain ischemia-reperfusion, and the decreasing of kinesin activity was greater than that of MAP2 in the same reperfusion time. Hypothermia obviously mitigate the decreasing degree of MAP2 and kinesin activity after cerebral ischemia. The decreasing degree of kinesin activity was consistent with that of DND during cerebral ischemia/reperfusion in gerbils. CONCLUSION: Hypothermia significantly reduced DND after cerebral ischemia, which might be related to the improvement of kinesin activity after cerebral ischemia reperfusion in gerbils.