Translational pausing ensures membrane targeting and cytoplasmic splicing of XBP1u mRNA.
Science
; 331(6017): 586-9, 2011 Feb 04.
Article
em En
| MEDLINE
| ID: mdl-21233347
ABSTRACT
Upon endoplasmic reticulum (ER) stress, an endoribonuclease, inositol-requiring enzyme-1α, splices the precursor unspliced form of X-box-binding protein 1 messenger RNA (XBP1u mRNA) on the ER membrane to yield an active transcription factor (XBP1s), leading to the alleviation of the stress. The nascent peptide encoded by XBP1u mRNA drags the mRNA-ribosome-nascent chain (R-RNC) complex to the membrane for efficient cytoplasmic splicing. We found that translation of the XBP1u mRNA was briefly paused to stabilize the R-RNC complex. Mutational analysis of XBP1u revealed an evolutionarily conserved peptide module at the carboxyl terminus that was responsible for the translational pausing and was required for the efficient targeting and splicing of the XBP1u mRNA. Thus, translational pausing may be used for unexpectedly diverse cellular processes in mammalian cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Biossíntese de Proteínas
/
RNA Mensageiro
/
Splicing de RNA
/
Citoplasma
/
Proteínas de Ligação a DNA
/
Retículo Endoplasmático
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2011
Tipo de documento:
Article