Protein and peptide fractionation, enrichment and depletion: tools for the complex proteome.
Proteomics
; 11(4): 513-34, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21241016
ABSTRACT
The identification, quantitation and global characterisation of all proteins within a given proteome are extremely challenging. This is due to the absolute detection limits of technology as well as the dynamic range in expression of proteins; and the extreme diversity and heterogeneity of the proteome. To overcome such issues, the use of separation technologies has played a critical role in reducing sample complexity. To date, a plethora of chromatographic and electrophoretic fractionation tools have evolved over the years assisting in simplifying complex protein and peptide mixtures. Here, we review a range of these technologies highlighting the challenges of protein and peptide analysis in the context of proteome research and some of the advantages and disadvantages of present techniques.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Técnicas de Química Analítica
/
Proteômica
Idioma:
En
Revista:
Proteomics
Ano de publicação:
2011
Tipo de documento:
Article