Effects of the molecular weight and the degree of deacetylation of chitosan oligosaccharides on antitumor activity.
Int J Mol Sci
; 12(1): 266-77, 2011 Jan 06.
Article
em En
| MEDLINE
| ID: mdl-21339986
ABSTRACT
Effects of the degree of deacetylation (DDA) and the molecular mass of chitosan oligosaccharides (CTS-OS), obtained from the enzymatic hydrolysis of high molecular weight chitosan (HMWC), on antitumor activity was explored. The DDA and molecular weights of CTS-OS were determined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-TOF MS) analysis. The CTS-OS were found to be a mixture of mainly dimers (18.8%), trimers (24.8%), tetramers (24.9%), pentamers (17.7%), hexamers (7.1%), heptamers (3.3%), and octamers (3.4%). The CTS-OS were further fractionated by gel-filtration chromatography into two major fractions (1) COS, consisting of glucosamine (GlcN)(n), n = 3-5 with DDA 100%; and (2) HOS, consisting of (GlcN)(5) as the minimum residues and varying number of N-acetylglucosamine (GlcNAc)(n), n = 1-2 with DDA about 87.5% in random order. The cytotoxicities, expressed as the concentration needed for 50% cell death (CC(50)), of CTS-OS, COS, and HOS against PC3 (prostate cancer cell), A549 (lung cancer cell), and HepG2 (hepatoma cell), were determined to be 25 µg·mL(-1), 25 µg·mL(-1), and 50 µg·mL(-1), respectively. The HMWC was approximately 50% less effective than both CTS-OS and COS. These results demonstrate that the molecular weight and DDA of chitosan oligosaccharides are important factors for suppressing cancer cell growth.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Quitosana
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Int J Mol Sci
Ano de publicação:
2011
Tipo de documento:
Article