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The chromogranin A- derived N-terminal peptide vasostatin-I: In vivo effects on cardiovascular variables in the rabbit.
Roatta, Silvestro; Passatore, Magda; Novello, Matteo; Colombo, Barbara; Dondossola, Eleonora; Mohammed, Mazher; Losano, Gianni; Corti, Angelo; Helle, Karen B.
Afiliação
  • Roatta S; Dept of Neurosciences, Physiology, Medical School, Torino, Italy.
Regul Pept ; 168(1-3): 10-20, 2011 Jun 07.
Article em En | MEDLINE | ID: mdl-21362443
ABSTRACT
This study is the first to report on vascular effect of the chromogranin A derived Vasostatin-I (CgA(1-76)) in vivo. Cardiovascular parameters were recorded in 29 rabbits with sympathetically decentralized right carotid vascular bed. The recombinant human STA CgA(1-78) (VS-1) was infused at 480 µg/kg over 25 min. Group I was kept awake while groups II-V were anesthetized with Ketamine-xylazine. VS-1 was given alone in groups I-II while in presence of either phentolamine, phentolamine plus propranolol or hexamethonium in groups III-V. Serum VS-1 peaked at 2 µg/ml (200 nM) before onset of vascular effects and declined rapidly to ~200 ng/ml within 30 min. In all groups but III and IV VS-1 induced a brief vasoconstriction, being larger in intact than in sympathetically decentralized beds. The VS-1 induced vasoconstriction was not altered by hexamethonium but was abolished by phentolamine. In presence of the α-adrenergic blocker a long lasting vasodilatation, unaffected by propranolol, was apparent on both innervated and decentralized sides. In conclusion, VS-1 induced an α-adrenoceptor-mediated vasoconstriction presumably brought about by noradrenaline release from sympathetic nerves when infused at a dose giving an initial serum concentration of ~200 nM. This initial vasoconstriction masked a persistent adrenoceptor-independent vasodilatation, consistent with previous reports from in vitro models.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Vasoconstrição / Fenômenos Fisiológicos Cardiovasculares / Cromogranina A Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Regul Pept Ano de publicação: 2011 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Vasoconstrição / Fenômenos Fisiológicos Cardiovasculares / Cromogranina A Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Regul Pept Ano de publicação: 2011 Tipo de documento: Article