Chromogranins A and B are co-localized with atrial natriuretic peptides in secretory granules of rat heart.
J Histochem Cytochem
; 38(6): 845-50, 1990 Jun.
Article
em En
| MEDLINE
| ID: mdl-2139887
ABSTRACT
We investigated the occurrence and subcellular localization of chromogranins A and B in atrial myoendocrine cells of rat heart, using immunological methods. Immunoblotting revealed the presence of both chromogranin A and B in an extract from large granules isolated from this tissue by subcellular fractionation. Immunohistochemistry at the ultrastructural level demonstrated the presence of chromogranin A and B in secretory granules. These organelles also immunostained for atrial natriuretic peptides (ANP). Within a given section, all granules were labeled with immunogold for these three antigens. This apparent co-localization of the three antigens was confirmed by double immunostaining with immunogold particles of different sizes. We conclude that, in agreement with their endocrine nature, the secretory organelles of rat atria contain both chromogranins A and B. Apparently these acidic peptides, which have a widespread distribution in the endocrine system, are co-stored and therefore also co-secreted with ANP.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromograninas
/
Fator Natriurético Atrial
/
Grânulos Citoplasmáticos
/
Miocárdio
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
J Histochem Cytochem
Ano de publicação:
1990
Tipo de documento:
Article