Protection of human muscle acetylcholinesterase from soman by pyridostigmine bromide.
Muscle Nerve
; 43(4): 591-5, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-21404290
ABSTRACT
INTRODUCTION:
Pretreatment with pyridostigmine bromide (PB) of human intercostal muscle fibers exposed to the irreversible acetylcholinesterase (AChE) inhibitor soman was investigated.METHODS:
Muscles were pretreated with 3 × 10(-6) M PB or saline for 20 minutes, then exposed to 10(-7) M soman for 10 minutes.RESULTS:
AChE of muscles treated with soman alone was inhibited >95%. In contrast, PB pretreatment of soman-exposed bundles protected 20% of AChE activity. AChE of bundles exposed to PB alone recovered after 4 hours, but bundles exposed to both PB and soman did not. Soman-induced reduction of resting membrane potentials and increment of amplitudes and decay times of miniature endplate potentials (MEPPs) were partially corrected by PB pretreatment.CONCLUSIONS:
In vitro pretreatment of human muscles with PB protected up to 20% of muscle AChE and ameliorated some deleterious effects on endplate physiology induced by soman.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcolinesterase
/
Brometo de Piridostigmina
/
Soman
/
Músculos Intercostais
/
Inibidores da Colinesterase
Limite:
Humans
Idioma:
En
Revista:
Muscle Nerve
Ano de publicação:
2011
Tipo de documento:
Article