Rapid isolation and chemical synthesis of two porcine cardiodilatins, the cardiac hormone precursor and related fragment.

To evaluate the chemically synthesized materials, two cardiodilatins, CDD-126 and CDD-88/CDD(39-126), a precursor of atrial hormone and a related fragment, were isolated from porcine atria by immunoaffinity chromatography or alginic acid adsorption followed by an ion exchange high performance liquid chromatography. The chemical synthesis was carried out using an automated peptide synthesizer. After cleavage and refolding, the crude CDDs were directly characterized by the novel method of primary structure determination using electroblotting and microsequencing. The purified synthetic CDDs were identical with the natural ones in physicochemical and immunochemical properties, as well as their biological actions both in vivo and in vitro.