Development and in vitro characterization of a bivalent DNA containing HN and F genes of velogenic Newcastle disease virus.
Indian J Exp Biol
; 49(2): 140-5, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21428216
ABSTRACT
Newcastle disease (ND) is highly contagious, economically important viral disease affecting most of avian species worldwide. Newcastle disease virus (NDV) has single stranded negative sense RNA genome which encodes for six structural and two non-structural proteins. Envelope glycoproteins i.e. hemagglutinin-neuraminidase (HN) and the fusion (F), elicit protective immune response. In this study, HN and F genes of velogenic (virulent) strain were amplified and cloned at multiple cloning sites A and B, respectively into pIRES bicistronic vector for use as bivalent DNA vaccine against ND. The recombinant plasmid was characterized for its orientation by restriction enzyme digestion and PCR. Expression of HN and F genes was assessed in transfected Vero cells at RNA level using RT-PCR in total RNA as well as protein level using IFAT, IPT and western blot using NDV specific antiserum. All these experiments confirmed that HN and F genes cloned in recombinant pIRES.nd.hn.f are functionally active. The recombinant construct is being evaluated as DNA vaccine against ND.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Doença de Newcastle
/
Proteína HN
/
Proteínas Virais de Fusão
/
Clonagem Molecular
/
Vacinas de DNA
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Indian J Exp Biol
Ano de publicação:
2011
Tipo de documento:
Article