The molecular basis of anaphase A in animal cells.

ABSTRACT

The mechanisms that move chromatids poleward during anaphase A have fascinated researchers for decades. There is now growing evidence that this movement is tightly linked to the active depolymerization of both ends of kinetochore-associated microtubules, a mechanism we refer to as "Pacman-Flux." Contemporary data suggest that this is catalyzed by the integration of multiple enzymatic activities including (1) microtubule-end depolymerases housed at the pole or kinetochore, (2) microtubule-severing enzymes used to uncap the ends of kinetochore-associated microtubules, and (3) molecular motors which drive tubulins towards the pole or into kinetochores.