Eop1 from a Rubus strain of Erwinia amylovora functions as a host-range limiting factor.
Phytopathology
; 101(8): 935-44, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21469934
ABSTRACT
Strains of Erwinia amylovora, the bacterium causing the disease fire blight of rosaceous plants, are separated into two groups based on host range Spiraeoideae and Rubus strains. Spiraeoideae strains have wide host ranges, infecting plants in many rosaceous genera, including apple and pear. In the field, Rubus strains infect the genus Rubus exclusively, which includes raspberry and blackberry. Based on comparisons of limited sequence data from a Rubus and a Spiraeoideae strain, the gene eop1 was identified as unusually divergent, and it was selected as a possible host specificity factor. To test this, eop1 genes from a Rubus strain and a Spiraeoideae strain were cloned and mutated. Expression of the Rubus-strain eop1 reduced the virulence of E. amylovora in immature pear fruit and in apple shoots. Sequencing the orfA-eop1 regions of several strains of E. amylovora confirmed that forms of eop1 are conserved among strains with similar host ranges. This work provides evidence that eop1 from a Rubus-specific strain can function as a determinant of host specificity in E. amylovora.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Regulação Bacteriana da Expressão Gênica
/
Rosaceae
/
Erwinia amylovora
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Phytopathology
Ano de publicação:
2011
Tipo de documento:
Article