TH1 and TH2 response to Campylobacter jejuni antigen in Guillain-Barre syndrome.
Arch Neurol
; 68(4): 445-52, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-21482924
ABSTRACT
OBJECTIVES:
To determine the expression of proinflammatory and anti-inflammatory cytokines in lymphocytes from the progressive and recovery phases of Guillain-Barré syndrome (GBS) after stimulation with Campylobacter jejuni outer membrane proteins.DESIGN:
Case-control study.SETTING:
Sanjay Gandhi Postgraduate Institute of Medical Sciences.PARTICIPANTS:
Sixty-five patients with GBS, 60 age- and sex-matched disease control individuals, and 68 healthy control individuals were included in the study. MAIN OUTCOMEMEASURES:
Lymphocytes from patients with GBS were stimulated with C jejuni outer membrane proteins, and the levels of different proinflammatory (T(H)1 [helper T cell, subtype 1]) and anti-inflammatory (T(H)2) cytokines were determined by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:
In the progressive phase of the disease, the expressions of interferon γ (IFN-γ), interleukin 1ß (IL-1ß), tumor necrosis factor (TNF), IL-6, IL-10, and the IFN-γIL-4 ratio were significantly upregulated, but expressions of transforming growth factor (TGF)-ß1 and IL-4 were lower in patients compared with disease and healthy controls. In contrast, the levels of IFN-γ, IL-1ß, TNF, IL-6, IL-10, and the IFN-γIL-4 ratio were significantly lower, but TGF-ß1 and IL-4 were upregulated in the recovery phase of GBS patients compared with controls.CONCLUSIONS:
Upregulation of T(H)1 cytokines in the early disease course may be associated with immune-mediated disease progression due to neuronal inflammation, but upregulation of T(H)2 immune response during the later phase aids recovery from the disease.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Campylobacter jejuni
/
Citocinas
/
Células Th2
/
Células Th1
/
Síndrome de Guillain-Barré
/
Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Adult
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Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Arch Neurol
Ano de publicação:
2011
Tipo de documento:
Article