Silver-stained fibrin zymography: separation of proteases and activity detection using a single substrate-containing gel.
Biotechnol Lett
; 33(8): 1663-6, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21487781
ABSTRACT
A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
/
Eletroforese em Gel de Poliacrilamida
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2011
Tipo de documento:
Article