Inactive fluorescently labeled xylanase as a novel probe for microscopic analysis of arabinoxylan containing cereal cell walls.
J Agric Food Chem
; 59(12): 6369-75, 2011 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-21561164
ABSTRACT
A new technique to visualize cereal cell walls by fluorescence microscopy was developed. The novel staining technique is based on an inactive fluorescently labeled xylanase binding to arabinoxylan (AX), an important polysaccharide in grain cell walls in terms of the technological and physiological functionalities of grain. The xylanase probe could stain AX in the seed coat, nucellar epidermis, aleurone layer, and starchy endosperm, but not the highly substituted AX of the pericarp layer. The advantage of this new staining technique over the existing immunolabeling techniques is that the staining procedure is clearly faster and less laborious, and uses a smaller probe that can easily be produced by marking a well characterized enzyme with a fluorescent label. In the future, the here proposed technology can be used to develop probes having specificity also for cell wall components other than AX and thus to study plant cell walls further through fluorescence microscopy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xilanos
/
Parede Celular
/
Grão Comestível
/
Xilano Endo-1,3-beta-Xilosidase
/
Microscopia de Fluorescência
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2011
Tipo de documento:
Article