Generation of a monoclonal antibody reactive to prefusion myocytes.
J Muscle Res Cell Motil
; 32(1): 31-8, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21597958
ABSTRACT
We established a novel monoclonal antibody, Yaksa that is specific to a subpopulation of myogenic cells. The Yaksa antigen is not expressed on the surface of growing myoblasts but only on a subpopulation of myogenin-positive myocytes. When Yaksa antigen-positive mononucleated cells were freshly prepared from a murine myogenic cell by a cell sorter, they fused with each other and formed multinucleated myotubes shortly after replating while Yaksa antigen-negative cells scarcely generated myotubes. These results suggest that Yaksa could segregate fusion-competent, mononucleated cells from fusion-incompetent cells during muscle differentiation. The Yaksa antigen was also expressed in developing muscle and regenerating muscle in vivo and it was localized at sites of cell-cell contact between mono-nucleated muscle cells and between mono-nucleated muscle cells and myotubes. Thus, Yaksa that marks prefusion myocytes before myotube formation can be a useful tool to elucidate the cellular and molecular mechanisms of myogenic cell fusion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desenvolvimento Muscular
/
Células Musculares
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Muscle Res Cell Motil
Ano de publicação:
2011
Tipo de documento:
Article