The opposing role of two prostaglandin D2 receptors, DP and CRTH2, in human eosinophil migration.
Ann Allergy Asthma Immunol
; 106(6): 511-7, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21624751
ABSTRACT
BACKGROUND:
Prostaglandin D2 (PGD2) regulates various immunological responses via two distinct PGD2 receptors, prostaglandin D receptor (DP), and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2). Recent studies have demonstrated that PGD2 induces the migration of eosinophils through CRTH2. Although human eosinophils express both DP and CRTH2, it is unclear whether the function of DP is involved in eosinophil migration.OBJECTIVE:
In this study, we investigated the roles of DP and CRTH2 in eosinophil migration by using selective agonists and antagonists.METHODS:
Eosinophils were isolated from human subjects with mild eosinophilia by modified CD16-negative selection. After stimulation with or without DP receptor agonist, eosinophil migration was measured by Boyden chamber. The effect of DP agonists on CRTH2-induced eosinophil migration was studied in terms of CRTH2 expression, Ca2+ mobilization, ERK/MAPK phosphorylation, and cyclic AMP (cAMP) production.RESULTS:
Treatment with DP agonists inhibited CRTH2-induced chemotaxis of eosinophils. Furthermore, we showed that DP agonists enhanced cAMP production in CRTH2 agonist stimulation without increasing CRTH2 expression. DP mediates eosinophils through the elevation of intracellular cAMP production but does not change CRTH2 expression.CONCLUSION:
Taken together, the balance between DP and CRTH2 could influence the degree of PGD2-induced eosinophil migration and DP agonist might regulate eosinophil activation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Prostaglandina
/
Receptores Imunológicos
/
Quimiotaxia
/
Eosinófilos
Limite:
Humans
Idioma:
En
Revista:
Ann Allergy Asthma Immunol
Ano de publicação:
2011
Tipo de documento:
Article