Site-specific chemical modification of a glycoprotein fragment expressed in yeast.
Methods Mol Biol
; 751: 329-42, 2011.
Article
em En
| MEDLINE
| ID: mdl-21674341
ABSTRACT
Site-specific modification of glycoproteins has wide application in both biochemical and biophysical studies. This method describes the conjugation of synthetic molecules to the N-terminus of a glycoprotein fragment, viz., human immunoglobulin G subclass 1 fragment crystallizable (IgG1 Fc), by native chemical ligation. The glycosylated IgG1 Fc is expressed in a glycosylation-deficient yeast strain. The N-terminal cysteine is generated by the endogenous yeast protease Kex2 in the yeast secretory pathway. The N-terminal cysteine is then conjugated with a biotin thioester to produce a biotinylated, glycosylated IgG1 Fc using native chemical ligation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Imunoglobulina G
/
Fragmentos Fc das Imunoglobulinas
/
Glicoproteínas
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2011
Tipo de documento:
Article