Study on the roles of ß-catenin in hydrogen peroxide-induced senescence in human skin fibroblasts.

ABSTRACT

Oxidative stress is one of the most important causes of the cellular senescence process. Previous studies showed that ß-catenin can regulate FoxO3a and this association was enhanced in cells exposed to oxidative stress. It has also been reported that ß-catenin can regulate some senescence-related proteins. We propose that ß-catenin may play a crucial role in senescence of normal human primary skin fibroblasts (NHSFs). Here, we explored the roles and mechanisms of ß-catenin on H(2)O(2)-induced senescence in NHSFs. ß-catenin expression was decreased in NHSFs after H(2)O(2) treatment. Overexpression of ß-catenin in NHSFs led to a marked delay of many senescent phenotypes induced by H(2)O(2). Furthermore, overexpression of ß-catenin in NHSFs can antagonise the alteration of reactive oxygen species accumulation and some senescence-related proteins expression induced by H(2)O(2) treatment. Our data demonstrated that ß-catenin can protect NHSFs from H(2)O(2)-induced premature senescence by alleviating oxidative stress and regulating some senescence-related molecules.