Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase.
J Chromatogr A
; 1218(31): 5362-8, 2011 Aug 05.
Article
em En
| MEDLINE
| ID: mdl-21726866
ABSTRACT
Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50 mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6±0.1 min (n=3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Celulose 1,4-beta-Celobiosidase
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2011
Tipo de documento:
Article