HIV-1 integrase inhibitor T30177 forms a stacked dimeric G-quadruplex structure containing bulges.
Nucleic Acids Res
; 39(20): 8984-91, 2011 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-21771859
ABSTRACT
T30177 is a G-rich oligonucleotide with the sequence (GTGGTGGGTGGGTGGGT) which inhibits the HIV-1 integrase activity at nanomolar concentrations. Here we show that this DNA sequence forms in K(+) solution a dimeric G-quadruplex structure comprising a total of six G-tetrad layers through the stacking of two propeller-type parallel-stranded G-quadruplex subunits at their 5'-end. All twelve guanines in the sequence participate in the G-tetrad formation, despite the interruption in the first G-tract by a thymine, which forms a bulge between two adjacent G-tetrads. In this work, we also propose a simple analytical approach to stoichiometry determination using concentration-dependent melting curves.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Inibidores de Integrase de HIV
/
Quadruplex G
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2011
Tipo de documento:
Article