Inhibitory effect of morin on DMH-induced biochemical changes and aberrant crypt foci formation in experimental colon carcinogenesis.

Morin is a flavonoid present in fruits and Chinese herbs, exhibits various beneficial biological activities. There are numerous evidence suggesting that total dietary fat intake is generally associated with early promotion of colon cancer, the alterations in the lipid profile is important for malignant transformation and tumor development and carbohydrate moieties of glycoproteins reflect the stage of cancer. Aberrant crypt foci (ACF) consisting of morphologically irregular crypts, are thought to be precancerous lesions for colon cancer. Our aim was to study the inhibitory effect morin on aberrant crypt foci and alterations in the levels of lipids, and glycoconjugates in experimental rat colon cancer. Group 1 served as control, groups 2 and 4 received 50mg/kg b.w. morin orally everyday for 30 weeks. Groups 3 and 4 were given subcutaneous injection of 1,2-dimethylhydrazine (DMH) 20mg/kg b.w. for the first 15 weeks. Administration of morin at the dose of 50mg/kg b.w., significantly suppressed the formation of ACF its multiplicity and lowered levels of serum and tissue lipids, cholesterol-phospholipid ratio, glycoconjugate and also increased the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase). These results indicate that morin has a protective effect against DMH-induced colon carcinogenesis.