Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats.
Parasitol Int
; 61(1): 203-7, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-21864712
ABSTRACT
The present study investigated the diagnostic value of an ELISA for the detection of Clonorchis sinensis antigen in the feces of experimentally infected rats. A mouse polyclonal IgG antibody against adult C. sinensis crude antigen (CsAg) was used to capture the C. sinensis coproantigen. The detection limit for pure CsAg was 20 ng/ml in sample buffer and 40 ng/ml in uninfected fecal extract. The test was evaluated using a follow-up of five groups of rats experimentally infected with 100, 50, 10, 5 and 1 metacercariae of C. sinensis and an uninfected control group. Coproantigen was detected in all infected groups of rats from 2 weeks of infection, whereas fecal eggs were not observed until 3 weeks of infection. As the infection period progressed, the fecal CsAg concentration increased in all groups of infected rats, even those infected with a single metacercaria. The fecal CsAg concentration was correlated positively with fecal egg counts and worm burden. This coproantigen capture ELISA is highly sensitive for the detection of CsAg in rat feces, and with further development, should be useful for mass screening of human subjects in clonorchiasis-endemic areas.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Clonorquíase
/
Clonorchis sinensis
/
Fezes
/
Antígenos de Helmintos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Prognostic_studies
/
Screening_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Parasitol Int
Ano de publicação:
2012
Tipo de documento:
Article