Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis.
Mol Syst Biol
; 7: 532, 2011 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-21952135
ABSTRACT
The heterotrimeric G-protein complex is minimally composed of Gα, Gß, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Parede Celular
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Arabidopsis
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Proteínas de Ligação ao GTP
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Proteínas de Arabidopsis
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Proteômica
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Receptores Acoplados a Proteínas G
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Glicômica
Idioma:
En
Revista:
Mol Syst Biol
Ano de publicação:
2011
Tipo de documento:
Article