The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States.
Clin Biochem
; 44(17-18): 1445-50, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21963384
ABSTRACT
OBJECTIVE:
We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples. DESIGN ANDMETHODS:
We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.RESULTS:
During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage.CONCLUSIONS:
Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triagem Neonatal
/
Teste em Amostras de Sangue Seco
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
/
Newborn
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Clin Biochem
Ano de publicação:
2011
Tipo de documento:
Article