Unexpected structure of a C. difficile toxin A ligand necessitates an annotation correction in a popular screening library.
Chem Commun (Camb)
; 47(45): 12397-9, 2011 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-22016886
ABSTRACT
The structure of the pentasaccharide S259-1 in the Consortium for Functional Glycomics was investigated using a variety of techniques. Surprisingly, the structure differs from the structure assumed from the previously established specificity of the human fucosyltransferase FUT-III used in the last step of chemoenzymatic synthesis. When presented with a tetrasaccharide substrate containing both type I and type II disaccharide moieties, the enzyme generates a pentasaccharide in which the type II moiety is preferentially fucosylated. The unexpected product generated by FUT-III in this case highlights the importance of performing detailed structural analysis on products generated by enzymes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Toxinas Bacterianas
/
Clostridioides difficile
/
Enterotoxinas
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Chem Commun (Camb)
Ano de publicação:
2011
Tipo de documento:
Article