Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices.
Methods Mol Biol
; 810: 73-88, 2012.
Article
em En
| MEDLINE
| ID: mdl-22057561
ABSTRACT
Nitric oxide (â¢NO) is a ubiquitous signaling molecule that participates in neuromolecular phenomena associated with memory formation as well as in excitotoxicity. In the hippocampus, neuronal â¢NO production is coupled to the activation of the NMDA-type of glutamate receptor. More recently, Cytochrome c oxidase has emerged as a novel target for â¢NO, which competes with O 2 for binding to this mitochondrial complex. This reaction establishes â¢NO not only as a regulator of cellular metabolism but possibly also as a regulator of mitochondrial production of reactive oxygen species which participate in cellular signaling. A major gap in the understanding of â¢NO bioactivity, namely, in the hippocampus, has been the lack of knowledge of its concentration dynamics. Here, we present a detailed description of the simultaneous recording of â¢NO and O2 concentration dynamics in rat hippocampal slices. Carbon fi ber microelectrodes are fabricated and applied for real-time measurements of both gases in a system close to in vivo models. This approach allows for a better understanding of the current paradigm by which an intricate interplay between â¢NO and O 2 regulates cellular respiration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hipocampo
/
Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2012
Tipo de documento:
Article