X-ray induced DNA double-strand breaks in coronary CT angiography: comparison of sequential, low-pitch helical and high-pitch helical data acquisition.
Eur J Radiol
; 81(3): e357-62, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22178288
ABSTRACT
BACKGROUND:
Aim of this study was to compare DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing high-pitch helical, low-pitch helical and sequential coronary CT angiography. METHODS ANDRESULTS:
66 patients were examined with various scan protocols and modes (low-pitch helical scan 100-120 kV, 320-438 mAs/rot, pitch 0.18-0.39, with or without ECG-pulsing, n=35; prospectively ECG-triggered high-pitch helical scan 100-120 kV, 320-456 mAs/rotation, pitch 3.2-3.4, n=19; prospectively ECG-triggered sequential scan 100-120 kV, 150-300 mAs or 320-370 mAs/rotation, n=12) either using a 64-slice or 128-slice dual-source CT or a 128-slice single source CT scanner. Blood samples were obtained before and 30 min after CT and DSBs were analyzed in isolated lymphocytes using γ-H2AX immunofluorescence microscopy. A significant increase of DSBs was measurable 30 min after CTA (range 0.01-0.71/cell). CT induced DSBs showed a significant correlation with the estimated effective dose (ρ=0.90, p<0.00001). Both prospectively ECG-triggered sequential (0.10 DSBs/cell, 176 mGy cm, p<0.00001) and high-pitch helical scan protocols (0.03 DSBs/cell, 109 mGy cm, p<0.00001) led to a significant reduction of median DLP and DSB levels compared to low-pitch helical scans (0.34 DSBs/cell, 828 mGy cm). A reduction of the tube voltage resulted in significantly lower whereas additional calcium scoring resulted in elevated DLP and DNA damages (p<0.05 each).CONCLUSION:
In coronary CTA, data acquisition protocols have a significant influence on the X-ray induced DSB levels. Using γ-H2AX immunofluorescence microscopy different scan modes in different CT generations can be compared concerning their biological impact.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
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Angiografia Coronária
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Doença das Coronárias
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Tomografia Computadorizada Espiral
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Quebras de DNA de Cadeia Dupla
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Técnicas de Imagem de Sincronização Cardíaca
Limite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Eur J Radiol
Ano de publicação:
2012
Tipo de documento:
Article