[The purification of tick-borne encephalitis virus preparations of cellular DNA]. / Ochistka preparatov virusa kleshchevogo éntsefalita ot kletochnoi DNK.
Vopr Virusol
; 35(3): 219-21, 1990.
Article
em Ru
| MEDLINE
| ID: mdl-2219855
ABSTRACT
According to the WHO requirements, the concentration of cellular DNA in vaccine preparations produced by pooling virus from continuous cell lines is limited to 100 ng/dose. In this study, different methods were used for purification of tick-borne encephalitis virus suspensions grown in continuous cultures of cell line 4647 from cellular DNA. Two approaches are proposed based on treatment with DNAse and promamin sulfate which allow one to reduce cellular DNA concentration in the virus preparation to the acceptable level. Prospects of their use in vaccine production are discussed.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
4_TD
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Vírus da Encefalite Transmitidos por Carrapatos
Limite:
Animals
Idioma:
Ru
Revista:
Vopr Virusol
Ano de publicação:
1990
Tipo de documento:
Article