CD4- and dynamin-dependent endocytosis of HIV-1 into plasmacytoid dendritic cells.
Virology
; 423(2): 152-64, 2012 Feb 20.
Article
em En
| MEDLINE
| ID: mdl-22209232
ABSTRACT
Chronic immune activation, triggered by plasmacytoid dendritic cell (PDC) interferon (IFN)-alpha production, plays an important role in HIV-1 pathogenesis. As the entry of HIV-1 seems to be important for the activation of PDC, we directly characterized the viral entry into these cells using immuno-electron microscopy, cellular fractionation, confocal imaging, and functional experiments. After attachment to PDC, viruses were taken up in an energy-dependent manner. The virions were located in compartments positive for caveolin; early endosomal antigen 1; Rab GTPases 5, 7 and 9; lysosomal-associated membrane protein 1. PDC harbored more virus in endocytic vesicles than CD4+ T cells (p<0.05). Blocking CD4 inhibited the uptake of virions into cytosolic and endosomal compartments. Dynasore, an inhibitor of dynamin-dependent endocytosis, not the fusion inhibitor T-20, reduced the HIV-1 induced IFN-alpha production. Altogether, our morphological and functional data support the role of endocytosis for the entry and IFN-alpha induction of HIV-1 in PDC.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Antígenos CD4
/
Infecções por HIV
/
HIV-1
/
Dinaminas
/
Endocitose
Limite:
Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2012
Tipo de documento:
Article