Effects of vitamin D-binding protein-derived macrophage-activating factor on human breast cancer cells.
Anticancer Res
; 32(1): 45-52, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22213287
ABSTRACT
BACKGROUND:
Searching for additional therapeutic tools to fight breast cancer, we investigated the effects of vitamin D-binding protein-derived macrophage activating factor (DBP-MAF, also known as GcMAF) on a human breast cancer cell line (MCF-7). MATERIALS ANDMETHODS:
The effects of DBP-MAF on proliferation, morphology, vimentin expression and angiogenesis were studied by cell proliferation assay, phase-contrast microscopy, immunohistochemistry and western blotting, and chorioallantoic membrane (CAM) assay.RESULTS:
DBP-MAF inhibited human breast cancer cell proliferation and cancer cell-stimulated angiogenesis. MCF-7 cells treated with DBP-MAF predominantly grew in monolayer and appeared to be well adherent to each other and to the well surface. Exposure to DBP-MAF significantly reduced vimentin expression, indicating a reversal of the epithelial/mesenchymal transition, a hallmark of human breast cancer progression.CONCLUSION:
These results are consistent with the hypothesis that the known anticancer efficacy of DBP-MAF can be ascribed to different biological properties of the molecule that include inhibition of tumour-induced angiogenesis and direct inhibition of cancer cell proliferation, migration and metastatic potential.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína de Ligação a Vitamina D
/
Neoplasias da Mama
/
Fatores Ativadores de Macrófagos
/
Proliferação de Células
/
Neovascularização Patológica
Limite:
Female
/
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2012
Tipo de documento:
Article