A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses.
Cell Microbiol
; 14(5): 669-81, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22233353
ABSTRACT
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His(6) -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant immunity responses and a target for pathogen suppression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fotossíntese
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Solanum lycopersicum
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Fatores de Virulência
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Pseudomonas syringae
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Complexo de Proteína do Fotossistema II
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Interações Hospedeiro-Patógeno
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Cisteína Proteases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Cell Microbiol
Ano de publicação:
2012
Tipo de documento:
Article