3-O-α-D-glucopyranosyl-L-rhamnose phosphorylase from Clostridium phytofermentans.
Carbohydr Res
; 350: 94-7, 2012 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-22277537
ABSTRACT
We found an unreported activity of phosphorylase catalyzed by a protein (Cphy1019) belonging to glycoside hydrolase family 65 (GH65) from Clostridium phytofermentans. The recombinant Cphy1019 produced in Escherichia coli did not phosphorolyze α-linked glucobioses, such as trehalose (α1-α1), kojibiose (α1-2), nigerose (α1-3), and maltose (α1-4), which are typical substrates for GH65 enzymes. In reverse phosphorolysis, Cphy1019 utilized only l-rhamnose as the acceptor among various sugars examined with ß-d-glucose 1-phosphate as the donor. The reaction product was determined to be 3-O-α-d-glucopyranosyl-l-rhamnose, indicating strict α1-3 regioselectivity. We propose 3-O-α-d-glucopyranosyl-l-rhamnose phosphate ß-d-glucosyltransferase as the systematic name and 3-O-α-d-glucopyranosyl-l-rhamnose phosphorylase as the short name for this novel GH65 phosphorylase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clostridium
/
Dissacarídeos
/
Glucosiltransferases
/
Fosforilases
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2012
Tipo de documento:
Article