Ultra acidic strong cation exchange enabling the efficient enrichment of basic phosphopeptides.
Anal Chem
; 84(4): 1804-8, 2012 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-22303813
ABSTRACT
We present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-represented class in common enrichment strategies. Our method is based on a two-dimensional strong cation exchange (SCX) strategy, operating at two different acidic pHs, enabling both separation and enrichment of different classes of phosphopeptides. The principle of enrichment is based on the change of net charge of phosphorylated peptides under strong acidic conditions in the second SCX, whereas the net charge of regular peptides remains unchanged, thus enabling separation based on net charge. Application of our tandem SCX approach to a modest amount of human cells allowed the identification of over 10,000 unique "basic" phosphopeptides of which many represent putative targets of basophilic kinases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfopeptídeos
/
Cátions
/
Cromatografia por Troca Iônica
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2012
Tipo de documento:
Article