Comparative analyses of protein complexes by blue native DIGE.
Methods Mol Biol
; 854: 145-54, 2012.
Article
em En
| MEDLINE
| ID: mdl-22311758
ABSTRACT
Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corantes de Rosanilina
/
Proteínas
/
Eletroforese em Gel Diferencial Bidimensional
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2012
Tipo de documento:
Article