Validation of Eupatorium triplinerve Vahl leaves, a skin care herb from East Kalimantan, using a melanin biosynthesis assay.
J Acupunct Meridian Stud
; 5(2): 87-92, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22483187
ABSTRACT
In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360 µM (L-tyrosine was used as the substrate) and above 2840 µM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780 µM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Cumarínicos
/
Eupatorium
/
Clareadores
/
Melaninas
Tipo de estudo:
Evaluation_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Acupunct Meridian Stud
Ano de publicação:
2012
Tipo de documento:
Article