Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells.
Plant Physiol Biochem
; 55: 77-84, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22554830
ABSTRACT
The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O×´-p-coumaroyl)-glucoside and peonidin-3-O-(6-O×´-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatos
/
Proteínas de Plantas
/
Regulação da Expressão Gênica de Plantas
/
Antocianinas
Idioma:
En
Revista:
Plant Physiol Biochem
Ano de publicação:
2012
Tipo de documento:
Article