A purified cellular extract accelerates the cell cycle in Physarum polycephalum.
Exp Cell Res
; 191(2): 332-6, 1990 Dec.
Article
em En
| MEDLINE
| ID: mdl-2257887
ABSTRACT
Plasmodia of the myxomycete Physarum polycephalum (strain Cl) were collected at different times during the cell cycle and extracts were prepared from homogenates using a buffer optimized for microinjection into plasmodial veins. These extracts were injected into plasmodia during the first 3 h of the cell cycle. The time of the following mitosis was monitored and compared with that of the buffer-injected controls. Extracts of plasmodia homogenized 45 min before late telophase accelerated the onset of mitosis in the injected plasmodium up to 70 min, i.e., an advance of 10-14% compared to the 8- to 10-h cell cycle duration of the controls. The accelerating activity vanished completely after heating, freezing, or protease digestion, thus indicating the peptide nature of the active agent. Purification of the active compound by means of gel filtration revealed a molecular mass of about 2500 Da. The active portion of the extract was further fractionated by HPLC and the activity determined in a single peak.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Physarum
/
Extratos Celulares
Limite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
1990
Tipo de documento:
Article