Analysis of reagent lot-to-lot comparability tests in five immunoassay items.
Ann Clin Lab Sci
; 42(2): 165-73, 2012.
Article
em En
| MEDLINE
| ID: mdl-22585613
ABSTRACT
We investigated the degree of lot-to-lot reagent variation for 5 common immunoassay items. We measured the commercial as well as in-house controls for α-fetoprotein (AFP), ferritin, CA19-9, quantitative hepatitis B surface antigen (HBsAg), and hepatitis B surface antibody (anti-HBs) 10 times each by using both the old and the new lot of reagents whenever a reagent lot was changed, over a period of 10 months. The differences in the mean control values, the percent difference (% difference), and the difference to between-run standard deviation ratio (DSD ratio) between successive lots were calculated. The % difference in mean control values between 2 reagent lots ranged from 0.1 to 17.5% for AFP, 1.0 to 18.6% for ferritin, 0.6 to 14.3% for CA19-9, 0.6 to 16.2% for HBsAg, and 0.1 to 17.7% for anti-HBs except negative controls of HBsAg and anti-HBs. The maximum DSD ratios between 2 lots were 4.37 for AFP, 4.39 for ferritin, 2.43 for CA19-9, 1.64 for HBsAg, and 4.16 for anti-HBs. Thus, we have experienced extensive variability in lot-to-lot reagent variation for 5 immunoassay items, indicating that reagent lot-to-lot comparability tests should be continuously performed and that laboratories should determine their own acceptance criteria for each item.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Imunoensaio
/
Indicadores e Reagentes
Limite:
Humans
Idioma:
En
Revista:
Ann Clin Lab Sci
Ano de publicação:
2012
Tipo de documento:
Article