Effective Genetic Expression of Nanoantibodies by Recombinant Adenoviral Vector in vitro.
Acta Naturae
; 3(3): 64-70, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-22649695
The present study is devoted to the feasibility of expressing the single-domain mini-antibody (nanoantibody) selected from the library of sequences of the variable domains of special single-stranded antibodies derived from an immunized camel, a gene of which was introduced into eukaryotic cells within a recombinant adenoviral vector. A vector bearing the gene of a single-domain nanoantibody was obtained using the AdEasy Adenoviral Vector System (Stratagene). This method of delivering the nanoantibody gene facilitates efficient expression of this gene and functional activity of the nanoantibody. The results obtained can be used to produce passive immunizing tools against pathogens or new-generation immunobiological antitoxic medication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Acta Naturae
Ano de publicação:
2011
Tipo de documento:
Article