Misregulation of Rad50 expression in melanoma cells.
J Cutan Pathol
; 39(7): 680-684, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22725639
ABSTRACT
DNA double-strand breaks are increased in human melanoma tissue as detected by histone H2AX phosphorylation.(1-3) We investigated two of the downstream effectors of DNA double-strand breaks, Rad50 and 53BP1 (tumor suppressor p53 binding protein 1), to determine if they are altered in human primary melanoma cells. Melanoma cases showed high Rad50 staining (81.8%; 9/11) significantly more frequently than conventional or atypical melanocytic nevi (0%; 0/18). In contrast, the staining pattern for 53BP1 appears similar between melanoma and nevi. This is the first study that shows activation and misregulation of the DNA repair pathway in human melanoma cells. The staining features of Rad50, a component of an essential DNA double-strand break repair complex, are clearly increased in melanoma cells with regards to both staining intensity and the number of positive melanoma cells. Interestingly, among the melanoma cases with increased Rad50 staining, most demonstrated cytoplasmic rather than nuclear staining (88.9%, 8/9). Further studies are needed to determine the cause of this mislocalization and its affects, if any, on DNA double-strand break repair in melanoma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Enzimológica da Expressão Gênica
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Regulação Neoplásica da Expressão Gênica
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Enzimas Reparadoras do DNA
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Proteínas de Ligação a DNA
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Melanoma
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Proteínas de Neoplasias
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Cutan Pathol
Ano de publicação:
2012
Tipo de documento:
Article