Hobi-like pestivirus: both biotypes isolated from a diseased animal.
J Gen Virol
; 93(Pt 9): 1976-1983, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22764319
ABSTRACT
A Hobi-like pestivirus pair consisting of cytopathogenic (cp) and non-cytopathogenic (noncp) strains, Italy 83/10cp and Italy 83/10ncp, was isolated from the lung of a heifer that died of respiratory disease. The noncp and cp viruses were isolated on Madin-Darby bovine kidney cells and separated by plaque purification and end point dilution. Analysis of the nearly full-length genomes revealed that the two viruses were very closely related to each other and to the noncp Hobi-like strain Italy 1/10-1, which had been isolated a few weeks earlier from the same herd. One major difference between noncp and cp viruses concerned the presence of a cellular Jiv sequence in the 3' domain of the NS2-encoding region of the cp strain. This is the first study, to our knowledge, reporting the isolation and molecular characterization of a Hobi-like virus pair.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
/
Infecções por Pestivirus
/
Pestivirus
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2012
Tipo de documento:
Article