In vivo emergence of Aspergillus terreus with reduced azole susceptibility and a Cyp51a M217I alteration.
J Infect Dis
; 206(6): 981-5, 2012 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-22782947
ABSTRACT
Azole resistance in Aspergillus terreus isolates was explored. Twenty related (MB) and 6 unrelated A. terreus isolates were included. CYP51A sequencing and RAPD genotyping was performed. Five MB isolates were itraconazole susceptible, whereas the minimum inhibitory concentrations (MICs) for 15 MB isolates were elevated (1-2 mg/L). Voriconazole and posaconazole MICs were 0.5-4 and 0.06-0.5 mg/L, respectively, for MB isolates but 0.25-0.5 and <0.03-0.06 mg/L, respectively, for controls. Sequencing identified a Cyp51Ap M217I alteration in all 15 isolates with elevated itraconazole MICs. Genotyping showed that 18 of 20 MB isolates were identical and unique, suggesting endogenous origin. In conclusion, itraconazole resistance in A. terreus was linked to an M217I Cyp51A alteration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus
/
Azóis
/
Proteínas Fúngicas
/
Sistema Enzimático do Citocromo P-450
/
Farmacorresistência Fúngica Múltipla
/
Antifúngicos
Limite:
Humans
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2012
Tipo de documento:
Article