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A replication-deficient rabies virus vaccine expressing Ebola virus glycoprotein is highly attenuated for neurovirulence.
Papaneri, Amy B; Wirblich, Christoph; Cann, Jennifer A; Cooper, Kurt; Jahrling, Peter B; Schnell, Matthias J; Blaney, Joseph E.
Afiliação
  • Papaneri AB; Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, MD 21702, USA.
Virology ; 434(1): 18-26, 2012 Dec 05.
Article em En | MEDLINE | ID: mdl-22889613
We are developing inactivated and live-attenuated rabies virus (RABV) vaccines expressing Ebola virus (EBOV) glycoprotein for use in humans and endangered wildlife, respectively. Here, we further characterize the pathogenesis of the live-attenuated RABV/EBOV vaccine candidates in mice in an effort to define their growth properties and potential for safety. RABV vaccines expressing GP (RV-GP) or a replication-deficient derivative with a deletion of the RABV G gene (RVΔG-GP) are both avirulent after intracerebral inoculation of adult mice. Furthermore, RVΔG-GP is completely avirulent upon intracerebral inoculation of suckling mice unlike parental RABV vaccine or RV-GP. Analysis of RVΔG-GP in the brain by quantitative PCR, determination of virus titer, and immunohistochemistry indicated greatly restricted virus replication. In summary, our findings indicate that RV-GP retains the attenuation phenotype of the live-attenuated RABV vaccine, and RVΔG-GP would appear to be an even safer alternative for use in wildlife or consideration for human use.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Vacina Antirrábica / Vacinas contra Ebola Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Virology Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Vacina Antirrábica / Vacinas contra Ebola Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Virology Ano de publicação: 2012 Tipo de documento: Article