Quantification of fiber orientation in the canine atrial pacemaker complex using optical coherence tomography.
J Biomed Opt
; 17(7): 071309, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22894470
ABSTRACT
The atrial pacemaker complex is responsible for the initiation and early propagation of cardiac impulses. Optical coherence tomography (OCT), a nondestructive imaging modality with spatial resolutions of â¼1 to 15 µm, can be used to identify unique fiber orientation patterns in this region of the heart. Functionally characterized canine sinoatrial nodes (SAN) (n=7) were imaged using OCT up to â¼1 mm below the endocardial tissue surface. OCT images were directly compared to their corresponding histological sections. Fiber orientation patterns unique to the crista terminalis (CT), SAN, and surrounding atrial myocardium were identified with dominant average fiber angles of 89 ± 12 deg, 110 ± 16 deg, and 95 ± 35 deg, respectively. Both the CT and surrounding atrial myocardium displayed predominantly unidirectionally based fiber orientation patterns within each specimen, whereas the SAN displayed an increased amount of fiber disarray manifested quantitatively as a significantly greater standard deviation in fiber angle distribution within specimens [33 ± 7 deg versus 23 ± 5 deg, atrium (p=0.02); 18 ± 3 deg, CT (p=0.0003)]. We also identified unique, local patterns of fiber orientation specific to the functionally characterized block zone. We demonstrate the ability of OCT in detecting components of the atrial pacemaker complex which are intimately involved in both normal and abnormal cardiac conduction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Relógios Biológicos
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Miócitos Cardíacos
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Tomografia de Coerência Óptica
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Átrios do Coração
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Sistema de Condução Cardíaco
Limite:
Animals
Idioma:
En
Revista:
J Biomed Opt
Ano de publicação:
2012
Tipo de documento:
Article