The localization and regulation of proprotein convertase subtilisin/kexin (PCSK) 6 in human ovary.
Am J Reprod Immunol
; 68(6): 491-8, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-22935039
ABSTRACT
PROBLEM:
The aim of this study is to evaluate the expression and regulation of proprotein convertase subtilisin/kexin (PCSK) 6, which is known to be an important factor in the production of bone morphogenetic protein (BMP) cytokines in human ovary. METHOD OF STUDY The localization of PCSK 6 protein in normal human ovaries was examined by immunohistochemistry. Human granulosa cells (GC), obtained from 34 patients undergoing ovarian stimulation for in vitro fertilization, were cultured with BMP-2, BMP-6, BMP-7, BMP-15, growth differentiation factor (GDF)-9, and activin-A with or without FSH. PCSK 6 mRNA expression level was evaluated by quantitative real-time reverse transcription and polymerase chain reaction (RT-PCR).RESULTS:
An immunohistochemistry study revealed that GC expressed PCSK 6 throughout follicular development, beginning in the primary follicle stage, while oocytes expressed PCSK 6 from the primordial follicle stage onwards. An in vitro study demonstrated that BMP-2, BMP-6, BMP-7, and BMP-15, not activin-A and GDF-9, decreased PCSK 6 gene expression in human GC. FSH induced PCSK 6 mRNA in the presence of activin-A or GDF-9. GDF-3, which is an inhibitor of BMP cytokines, also induced PCSK 6 mRNA expression.CONCLUSIONS:
PCSK 6, which is a critical factor to produce BMP cytokines, was suppressed with BMP stimulation in human GC, suggesting the presence of a negative feedback system in the follicular development process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ovário
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Serina Endopeptidases
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Proteínas Morfogenéticas Ósseas
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Ativinas
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Pró-Proteína Convertases
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Fator 9 de Diferenciação de Crescimento
Limite:
Adult
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Female
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Humans
Idioma:
En
Revista:
Am J Reprod Immunol
Ano de publicação:
2012
Tipo de documento:
Article