Automated DNA extraction for large numbers of plant samples.
Methods Mol Biol
; 938: 139-45, 2013.
Article
em En
| MEDLINE
| ID: mdl-22987412
ABSTRACT
The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
DNA de Plantas
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2013
Tipo de documento:
Article