[EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata].
Zhongguo Zhong Yao Za Zhi
; 37(12): 1731-3, 2012 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-22997813
ABSTRACT
OBJECTIVE:
To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata.METHOD:
Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized. RESULT ANDCONCLUSION:
The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Pinellia
/
Metanossulfonato de Etila
/
Mutação
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Ano de publicação:
2012
Tipo de documento:
Article