Measuring direct thrombin inhibitors with routine and dedicated coagulation assays: which assay is helpful?
Am J Clin Pathol
; 138(4): 551-8, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-23010710
ABSTRACT
The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatroban, and bivalirudin) in 6 pooled plasma specimens on routine coagulation assays (activated partial thromboplastin time [aPTT], prothrombin time [PT], and thrombin time [TT]) and dedicated DTI assays (Hemoclot, HemosIL, the ecarin clotting time, and a chromogenic ecarin clotting time) on 2 coagulation analyzers. We found routine tests to be nondiscriminative between concentrations of different DTIs in the aPTT. Moreover, for PT and TT, the responses for different DTIs differed. This was similar for ecarin clotting assays. The Hemoclot and HemosIL assays showed identical linear increases for all 3 DTIs. We conclude that dedicated calibrated assays based on a diluted TT (Hemoclot and HemosIL) appear to be the most suitable for monitoring purposes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Ácidos Pipecólicos
/
Coagulação Sanguínea
/
Trombina
/
Antitrombinas
/
Hirudinas
Limite:
Humans
Idioma:
En
Revista:
Am J Clin Pathol
Ano de publicação:
2012
Tipo de documento:
Article