Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR.
Mol Ecol Resour
; 13(1): 96-102, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23039969
ABSTRACT
Leafminer flies, especially, Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii, are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR, we newly sequenced the mitochondrial cytochrome oxidase I (COI) genes of Liriomyza bryoniae, Liriomyza chinensis, L. huidobrensis, L. sativae, L. trifolii, Chromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases (DDBJ/EMBL/GenBank). We then designed species-specific primers to allow us to differentiate between L. bryoniae, L. chinensis, L. huidobrensis, L. sativae, and L. trifolii.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Primers do DNA
/
Dípteros
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Health_economic_evaluation
Limite:
Animals
Idioma:
En
Revista:
Mol Ecol Resour
Ano de publicação:
2013
Tipo de documento:
Article