Chlorosulfonation of polystyrene substrates for bioanalytical assays: distribution of activated groups at the surface.
Analyst
; 137(23): 5666-71, 2012 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-23061089
ABSTRACT
In this work the activation of transparent PS substrates by chlorosulfonation is described and their distribution in the subsurface region is analyzed. For this purpose XPS, FTIR-ATR and colorimetry have been used. It is shown that the electrophilic aromatic substitution of polystyrene in pure chlorosulfonic acid is extremely quick with complete surface coverage by chlorosulfonic groups achieved after only a 10 minute reaction time at -10 °C. It is further demonstrated that the reaction is very surface selective and that even after reaction times as long as 3 hours, the modification is limited to a layer with a thickness of less than one micron. The activated PS substrates can be further functionalized in a second step with carboxylic groups. Due to the excellent optical transparency that the samples maintain upon modification, the modified systems were successfully probed for use in ELISA assays.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliestirenos
/
Ácidos Sulfônicos
/
Propriedades de Superfície
Idioma:
En
Revista:
Analyst
Ano de publicação:
2012
Tipo de documento:
Article